Hemocytometer

The hemocytometer is a medical counting-chamber device originally designed and usually used for counting blood cells.

Hemocytometer   - image1The hemocytometer was invented by Louis-Charles Malassez in 1874 and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber. This chamber is engraved with a laser-etched, glass slide grid of perpendicular lines. This grid is further divided into smaller and smaller cells.

The device is carefully crafted so the area bounded by the lines is known, and the depth of the chamber is also known. By observing a defined area of the grid, it is possible to count the number of cells or particles in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall. 

Hemocytometer   - homecytometerHospitals did use the hemocytometer to count blood cells. It is a conventional method of placing the smear under microscope and counting the cells manually, Since advances in computers hardware and software, new instruments as (Automated body fluid (BF) machines , have been steadily replacing manual hemocytometer (counting chamber) in clinical laboratories.

Enlargement of a glass microscope slide.    

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